Underground isoleucine biosynthesis pathways in E. coli

Biosynthesis of Valine and Isoleucine

The isotope studies of Strassman et al. (3, 4) first implicated cu-acetolactate and cu-aceto-cY-hydroxybutyrate as biosynthetic precursors of valine and isoleucine, respectively. The observed accumulation of acetolactate by Escherichia coli (5, 6) and of the decarboxylation products of acetolactate and acetohydroxybutyrate by Neurospora crassa (7) when mutants of these organisms are grown with ...

Biosynthesis of Valine and Isoleucine

Evidence published in recent years has shown that the biosynthesis of valine and isoleucine proceeds by a similar sequence of reactions (24). Fig. 1 depicts the reactions considered to be involved. In this report only Reactions II, III, and IV will be considered. Reaction II, the isomerization of the Lu-hydroxy$keto acid (ac-acetolactate for valine biosynthesis and a-aceto-a-hydroxybutyrate for...

Biosynthesis of β-carotene in engineered E. coli using the MEP and MVA pathways

BACKGROUND β-carotene is a carotenoid compound that has been widely used not only in the industrial production of pharmaceuticals but also as nutraceuticals, animal feed additives, functional cosmetics, and food colorants. Currently, more than 90% of commercial β-carotene is produced by chemical synthesis. Due to the growing public concern over food safety, the use of chemically synthesized β-c...

Multiple pathways of putrescine biosynthesis in Escherichia coli.

Nanotoxicity for E. Coli and Characterization of Silver Quantum Dots Produced by Biosynthesis with Eichhornia crassipes

Nanomaterials are widely used in health and biomedical applications, however, only a few studies investigate their toxic effects.  The present report signifies a contribution to the study of the toxic effects of silver nanoparticles on   E. coli cells, which is a model organism of anthropogenic pollution. The toxicity of nanoparticles depends on their chemical and surface properties, shape and ...